價格
￥1,680.00
型號
RG011,OD104
品牌
Pickering/皮克林
所在地
天津市 南開區(qū)
更新時間
2020-08-26 12:40:04
瀏覽次數(shù)
次
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RG011,OD104,皮克林/Pickering柱后衍生
OPA 用于衍生分析氨基酸是1971年首次提出。圖 1 為 OPA 與一級氨基酸的反應(yīng)方程式。
室溫下， OPA （Pickering O120） 在巰基乙醇(Pickering 3710-2000)環(huán)境中與一級氨基酸于 1 min 內(nèi)即可生成具有強熒光的異吲哚衍生產(chǎn)物。OPA 本身不發(fā)熒光的特性使其在色譜分離時不形成干擾峰。OPA 只能與經(jīng)次氯酸鈉等氧化劑氧化開環(huán)后的二級氨基酸反應(yīng)，也可與 FMOC － Cl 聯(lián)用衍生二級氨基酸。OPA 衍生法因樣品制備簡單、衍生反應(yīng)迅速、靈敏度高及易于實現(xiàn)自動化操作等優(yōu)點而成為柱前衍生反相高效液相色譜法中使用廣泛的方法。
OPA 衍生法（用于檢測一級氨基酸）
試劑： 300 mg OPA （Pickering O120）, 2 g Thiofluor? (Pickering 3710-2000), 3 mL 30 % Brij® 35 溶于 950 mL OPA稀釋液(Pickering OD104)中
反應(yīng)器： 45 °C, 0.15 mL
衍生試劑流速： 0.3 mL/min
檢測器： 熒光檢測器
λex= 330 nm, λem=465 nm
相關(guān)產(chǎn)品目錄
0352-0021
30-MIN HIGH EFFICIENCY OXIDIZED FEED HYDROLYSATE KIT:
1154110T
30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-0070 amino acid test mixture
1700-3102
Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220
Sodium Diluent, pH 2.20, 4 x 250 mL
Na270
Sodium Eluant, pH 2.80, 4 x 950 mL
Na425
Sodium Eluant, pH 4.25, 4 x 950 mL
Na640
Sodium Eluant, pH 6.40, 4 x 950 mL
RG011
Sodium Column Regenerant, 950 mL
T200
TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
1700-0155
Sodium Calibration Standard, for oxidized feed hydrolysate, 0.25 μmole/mL, 5 mL
0352-0022
KIT IDENTICAL TO 0352-0021 WITH ITEMS BELOW REPLACING T200
OD104
OPA Diluent, 4 x 950 mL
O120
o- Phthalaldehyde, 5 g
3700-2000
Thiofluor?, 10 g (2 each per kit)
55 分鐘高效分析包
0352-0031
HIGH-EFFICIENCY 55-MIN PROTEIN HYDROYSIS KIT:
1154150T
Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102
Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220
Sodium Diluent, pH 2.20, 4 x 250 mL
Na315
Sodium Eluant, pH 3.15, 4 x 950 mL
Na740
Sodium Eluant, pH 7.40, 4 x 950 mL
RG011
Sodium Column Regenerant, 950 mL
T200
TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506H
Sodium Calibration Standard, for protein hydrolysate, 0.25 μmole/mL, 5 mL
AO31FH
KIT IDENTICAL TO 0352-0031 WITH ITEMS BELOW REPLACING A T200
OD104
OPA Diluent, 4 x 950 mL
O120
o- Phthalaldehyde, 5 g
3700-2000
Thiofluor?, 10 g (2 each per kit)
0352-0032
HIGH-EFFICIENCY 55-MIN COLLAGEN HYDROLYSATE KIT
1154150T
Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102
Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220
Sodium Diluent, pH 2.20, 4 x 250 mL
Na315
Sodium Eluant, pH 3.15, 4 x 950 mL
Na740
Sodium Eluant, pH 7.40, 4 x 950 mL
RG011
Sodium Column Regenerant, 950 mL
T200
TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506C
Sodium Calibration Standard, for collagen hydrolysate 0.25 μmole/mL, Proline and Hydroxyproline, 1.25
μmole/mL, 5 mL
AO32FC
KIT IDENTICAL TO 0352-0032 WITH ITEMS BELOW REPLACING A T200
OD104
OPA Diluent, 4 x 950 mL
O120
o- Phthalaldehyde, 5 g
3700-2000
Thiofluor?, 10 g (2 each per kit)
0352-0017
60-MIN OXIDIZED FEED HYDROLYSATE KIT:
1154150T
Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102
Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220
Sodium Diluent, pH 2.20, 4 x 250 mL
Na270
Sodium Eluant, pH 2.80, 4 x 950 mL
Na740
Sodium Eluant, pH 7.40, 4 x 950 mL
RG011
Sodium Column Regenerant, 950 mL
T200
TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
1700-0155
Sodium Calibration Standard, for oxidized feed hydrolysate, 0.25 μmole/mL, 5 mL
0352-0019
KIT IDENTICAL TO 0352-0017 WITH ITEMS BELOW REPLACING T200
OD104
OPA Diluent, 4 x 950 mL
O120
o-Phthalaldehyde, 5 g
3700-2000
Thiofluor?, 10 g, (2 each per kit)
60 分鐘標準分析包
0352-0030
STANDARD 60-MIN PROTEIN HYDROLYSATE KIT
1193250
Sodium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070)
1700-3102
Cation-exchange GARD? assembly: Holder w/ 2 replaceable GARDs?
Na220
Sodium Diluent, pH 2.20, 4 x 250 mL
Na328
Sodium Eluant, pH 3.28, 4 x 950 mL
Na740
Sodium Eluant, pH 7.40, 4 x 950 mL
RG011
Sodium Column Regenerant, 950 mL
T200
TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506H
Sodium Calibration Standard, for protein hydrolysate, 0.25 μmole/mL, 5 mL
AO30SH
KIT IDENTICAL TO 0352-0030 WITH ITEMS BELOW REPLACING T100C
OD104
OPA Diluent, 4 x 950 mL
O120
o-Phthalaldehyde, 5 g
3700-2000
Thiofluor?, 10 g, (2 each per kit)
標準品&測試品
012506C
Collagen Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
L,L & allo-Hydroxylysine 4-trans-L-Hydroxyproline(1.25)
012506H
Protein Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
Leucine Lysine
1700-0155
Oxidized Feed Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
Leucine Lysine
1700-0070
Amino Acid Test Mixture Concentration 0.25 μmole/mL in 0.01 NHCl, 5.0 mL
Cysteic Acid Threonine Serine
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